A small molecular fluorescence probe capable of real-time monitoring the open and closed states of TRPA1 channel

Transient receptor potential ankyrin 1 (TRPA1) channel serves as a critical mediator of nociceptive signaling across neural circuits, modulating pathophysiological processes from asthma to neurogenic inflammation through its unique ability to integrate chemical, thermal, and mechanical stimuli. Real-time monitoring conformational dynamics of TRPA1 is of significance for dissecting the roles of TRPA1 channel. Here, we present a small molecular fluorescence probe FPTRP-1 featuring a state-dependent fluorescence signature that directly correlates with TRPA1 gating status. Confocal imaging verifies that the probe FPTRP-1 has high selectivity for TRPA1 channel, and can monitor the on-off states of TRPA1 in real time, providing a potential pharmacological tool to further elucidate the physiological and pathological roles of TRPA1 channel in biological systems.