Assembling Two-Phase Enzymatic Cascade Pathways in Pickering Emulsion

The design of enzymatic cascade pathways in biphasic systems with high efficiency is still a big challenge. Herein, we report a simple and efficient strategy for one-pot biphasic tandem reactions by positioning distinct biocatalysts in spatially separate domains of Pickering emulsion. Due to the large interfacial area and the stability of Pickering interfacial biocatalysts, the as-obtained Pickering emulsion system was confirmed as a nice microreactor with highly enhanced catalytic performance. Furthermore, in this hybrid catalytic system, unconventional computing relied on enzymatic cascade reactions can be performed. Such Pickering emulsion-based logic system offers several distinctive advantages, including less “cross-talk” and signal amplification. We believe that our result will have critical applications in future biocatalysis, nanomachines, smart diagnostics, intelligent drug delivery, nanomechanical, and electronics.