Biosensors: Homogeneous Detection

Hiroshi Ueda; Jinhua Dong

doi:10.1016/B978-0-12-822548-6.00053-4

Biosensors: Homogeneous Detection

Hiroshi Ueda
, Jinhua Dong

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

2 Scopus citations

Abstract

Unlike heterogeneous assays that need separation steps to obtain target-dependent signal, homogeneous assays can detect target molecules without tedious separation steps by using specialized reporters. In this chapter, principles to realize homogeneous detection and their applications to biosensing are reviewed. Especially, examples in immunosensing are introduced to realize rapid and handy detection of small molecule antigens in a noncompetitive and sensitive manner.

Original language	English
Title of host publication	Encyclopedia of Sensors and Biosensors
Subtitle of host publication	Volume 1-4, First Edition
Publisher	Elsevier
Pages	458-477
Number of pages	20
Volume	1-4
ISBN (Electronic)	9780128225486
ISBN (Print)	9780128225493
DOIs	https://doi.org/10.1016/B978-0-12-822548-6.00053-4
State	Published - 1 Jan 2022
Externally published	Yes

Keywords

Absorbance
Allosteric enzyme
Chemiluminescence
Fluorescence
Green fluorescent protein
Heterogeneous assay
Homogeneous assay
Multimeric enzyme
Noncompetitive detection
Open sandwich immunoassay
Protein-fragment complementation assay
Q-body
Quenchbody
Signal-on
Wash-free

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10.1016/B978-0-12-822548-6.00053-4

Cite this

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title = "Biosensors: Homogeneous Detection",

abstract = "Unlike heterogeneous assays that need separation steps to obtain target-dependent signal, homogeneous assays can detect target molecules without tedious separation steps by using specialized reporters. In this chapter, principles to realize homogeneous detection and their applications to biosensing are reviewed. Especially, examples in immunosensing are introduced to realize rapid and handy detection of small molecule antigens in a noncompetitive and sensitive manner.",

keywords = "Absorbance, Allosteric enzyme, Chemiluminescence, Fluorescence, Green fluorescent protein, Heterogeneous assay, Homogeneous assay, Multimeric enzyme, Noncompetitive detection, Open sandwich immunoassay, Protein-fragment complementation assay, Q-body, Quenchbody, Signal-on, Wash-free",

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TY - CHAP

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T2 - Homogeneous Detection

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AU - Dong, Jinhua

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N2 - Unlike heterogeneous assays that need separation steps to obtain target-dependent signal, homogeneous assays can detect target molecules without tedious separation steps by using specialized reporters. In this chapter, principles to realize homogeneous detection and their applications to biosensing are reviewed. Especially, examples in immunosensing are introduced to realize rapid and handy detection of small molecule antigens in a noncompetitive and sensitive manner.

AB - Unlike heterogeneous assays that need separation steps to obtain target-dependent signal, homogeneous assays can detect target molecules without tedious separation steps by using specialized reporters. In this chapter, principles to realize homogeneous detection and their applications to biosensing are reviewed. Especially, examples in immunosensing are introduced to realize rapid and handy detection of small molecule antigens in a noncompetitive and sensitive manner.

KW - Absorbance

KW - Allosteric enzyme

KW - Chemiluminescence

KW - Fluorescence

KW - Green fluorescent protein

KW - Heterogeneous assay

KW - Homogeneous assay

KW - Multimeric enzyme

KW - Noncompetitive detection

KW - Open sandwich immunoassay

KW - Protein-fragment complementation assay

KW - Q-body

KW - Quenchbody

KW - Signal-on

KW - Wash-free

UR - https://www.scopus.com/pages/publications/85146536639

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DO - 10.1016/B978-0-12-822548-6.00053-4

M3 - 章节

AN - SCOPUS:85146536639

SN - 9780128225493

VL - 1-4

SP - 458

EP - 477

BT - Encyclopedia of Sensors and Biosensors

PB - Elsevier

ER -

Biosensors: Homogeneous Detection

Abstract

Keywords

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