Blood-brain barrier dysfunction in mice induced by lipopolysaccharide is attenuated by dapsone

Ting Zhou; Lei Zhao; Rui Zhan; Qihua He; Yawei Tong; Xiaosheng Tian; Hecheng Wang; Tao Zhang; Yaoyun Fu; Yang Sun; Feng Xu; Xiangyang Guo; Dongsheng Fan; Hongbin Han; Dehua Chui

doi:10.1016/j.bbrc.2014.09.093

Blood-brain barrier dysfunction in mice induced by lipopolysaccharide is attenuated by dapsone

Ting Zhou
, Lei Zhao
, Rui Zhan
, Qihua He
, Yawei Tong
, Xiaosheng Tian
, Hecheng Wang
, Tao Zhang
, Yaoyun Fu
, Yang Sun
, Feng Xu
, Xiangyang Guo
, Dongsheng Fan
, Hongbin Han
, Dehua Chui

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Blood-brain barrier (BBB) dysfunction is a key event in the development of many central nervous system (CNS) diseases, such as septic encephalopathy and stroke. 4,4′-Diaminodiphenylsulfone (DDS, Dapsone) has displayed neuroprotective effect, but whether DDS has protective role on BBB integrity is not clear. This study was designed to examine the effect of DDS on lipopolysaccharide (LPS)-induced BBB disruption and oxidative stress in brain vessels. Using in vivo multiphoton imaging, we found that DDS administration significantly restored BBB integrity compromised by LPS. DDS also increased the expression of tight junction proteins occludin, zona occludens-1 (ZO-1) and claudin-5 in brain vessels. Level of reactive oxygen species (ROS) was reduced by DDS treatment, which may due to decreased nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and NOX2 expression. Our results showed that LPS-induced BBB dysfunction could be attenuated by DDS, indicated that DDS has a therapeutic potential for treating CNS infection and other BBB related diseases.

Original language	English
Pages (from-to)	419-424
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	453
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2014.09.093
State	Published - 24 Oct 2014
Externally published	Yes

Keywords

Blood-brain barrier integrity
Dapsone
Lipopolysaccharide

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10.1016/j.bbrc.2014.09.093

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title = "Blood-brain barrier dysfunction in mice induced by lipopolysaccharide is attenuated by dapsone",

abstract = "Blood-brain barrier (BBB) dysfunction is a key event in the development of many central nervous system (CNS) diseases, such as septic encephalopathy and stroke. 4,4′-Diaminodiphenylsulfone (DDS, Dapsone) has displayed neuroprotective effect, but whether DDS has protective role on BBB integrity is not clear. This study was designed to examine the effect of DDS on lipopolysaccharide (LPS)-induced BBB disruption and oxidative stress in brain vessels. Using in vivo multiphoton imaging, we found that DDS administration significantly restored BBB integrity compromised by LPS. DDS also increased the expression of tight junction proteins occludin, zona occludens-1 (ZO-1) and claudin-5 in brain vessels. Level of reactive oxygen species (ROS) was reduced by DDS treatment, which may due to decreased nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and NOX2 expression. Our results showed that LPS-induced BBB dysfunction could be attenuated by DDS, indicated that DDS has a therapeutic potential for treating CNS infection and other BBB related diseases.",

keywords = "Blood-brain barrier integrity, Dapsone, Lipopolysaccharide",

author = "Ting Zhou and Lei Zhao and Rui Zhan and Qihua He and Yawei Tong and Xiaosheng Tian and Hecheng Wang and Tao Zhang and Yaoyun Fu and Yang Sun and Feng Xu and Xiangyang Guo and Dongsheng Fan and Hongbin Han and Dehua Chui",

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doi = "10.1016/j.bbrc.2014.09.093",

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T1 - Blood-brain barrier dysfunction in mice induced by lipopolysaccharide is attenuated by dapsone

AU - Zhou, Ting

AU - Zhao, Lei

AU - Zhan, Rui

AU - He, Qihua

AU - Tong, Yawei

AU - Tian, Xiaosheng

AU - Wang, Hecheng

AU - Zhang, Tao

AU - Fu, Yaoyun

AU - Sun, Yang

AU - Xu, Feng

AU - Guo, Xiangyang

AU - Fan, Dongsheng

AU - Han, Hongbin

AU - Chui, Dehua

PY - 2014/10/24

Y1 - 2014/10/24

N2 - Blood-brain barrier (BBB) dysfunction is a key event in the development of many central nervous system (CNS) diseases, such as septic encephalopathy and stroke. 4,4′-Diaminodiphenylsulfone (DDS, Dapsone) has displayed neuroprotective effect, but whether DDS has protective role on BBB integrity is not clear. This study was designed to examine the effect of DDS on lipopolysaccharide (LPS)-induced BBB disruption and oxidative stress in brain vessels. Using in vivo multiphoton imaging, we found that DDS administration significantly restored BBB integrity compromised by LPS. DDS also increased the expression of tight junction proteins occludin, zona occludens-1 (ZO-1) and claudin-5 in brain vessels. Level of reactive oxygen species (ROS) was reduced by DDS treatment, which may due to decreased nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and NOX2 expression. Our results showed that LPS-induced BBB dysfunction could be attenuated by DDS, indicated that DDS has a therapeutic potential for treating CNS infection and other BBB related diseases.

AB - Blood-brain barrier (BBB) dysfunction is a key event in the development of many central nervous system (CNS) diseases, such as septic encephalopathy and stroke. 4,4′-Diaminodiphenylsulfone (DDS, Dapsone) has displayed neuroprotective effect, but whether DDS has protective role on BBB integrity is not clear. This study was designed to examine the effect of DDS on lipopolysaccharide (LPS)-induced BBB disruption and oxidative stress in brain vessels. Using in vivo multiphoton imaging, we found that DDS administration significantly restored BBB integrity compromised by LPS. DDS also increased the expression of tight junction proteins occludin, zona occludens-1 (ZO-1) and claudin-5 in brain vessels. Level of reactive oxygen species (ROS) was reduced by DDS treatment, which may due to decreased nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activity and NOX2 expression. Our results showed that LPS-induced BBB dysfunction could be attenuated by DDS, indicated that DDS has a therapeutic potential for treating CNS infection and other BBB related diseases.

KW - Blood-brain barrier integrity

KW - Dapsone

KW - Lipopolysaccharide

UR - https://www.scopus.com/pages/publications/84918578607

U2 - 10.1016/j.bbrc.2014.09.093

DO - 10.1016/j.bbrc.2014.09.093

M3 - 文章

C2 - 25268765

AN - SCOPUS:84918578607

SN - 0006-291X

VL - 453

SP - 419

EP - 424

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Blood-brain barrier dysfunction in mice induced by lipopolysaccharide is attenuated by dapsone

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Keywords

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