TY - JOUR
T1 - Catalytic hairpin assembly indirectly covalent on Fe3O4@C nanoparticles with signal amplification for intracellular detection of miRNA
AU - Fan, Yaofang
AU - Liu, Yanming
AU - Zhou, Qihui
AU - Du, Hao
AU - Zhao, Xueyang
AU - Ye, Fei
AU - Zhao, Huimin
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/2/1
Y1 - 2021/2/1
N2 - Fluorescence resonance energy transfer, a promising method for in situ imaging of miRNA in living cells, has intrinsic limitation on sensitivity and selectivity. Herein, a fluorescent amplification strategy based on catalyzed hairpin assembly indirectly covalent on Fe3O4@C nanoparticles via short single-stranded DNA was investigated for cellular miRNA detection in living cells, integrating non-enzyme target-active releasing for amplifying the signal output, highly quenching efficiency of Fe3O4@C nanoparticles with low background, ssDNA assisted fluorescent group-fueled chain releasing from Fe3O4@C nanoparticles with enhanced fluorescence response. The designed platform exhibits highly sensitive in a wide linear concentration range of 0.450 pM–190 pM and is highly specific for miRNA-20a detection with the ability of discriminating one mistake base. Additionally, the CHA-Fe3O4@C was successfully applied in imaging visualization of miRNA-20a in the living cell. The strategy provides a promising bioassay approach for clinical research.
AB - Fluorescence resonance energy transfer, a promising method for in situ imaging of miRNA in living cells, has intrinsic limitation on sensitivity and selectivity. Herein, a fluorescent amplification strategy based on catalyzed hairpin assembly indirectly covalent on Fe3O4@C nanoparticles via short single-stranded DNA was investigated for cellular miRNA detection in living cells, integrating non-enzyme target-active releasing for amplifying the signal output, highly quenching efficiency of Fe3O4@C nanoparticles with low background, ssDNA assisted fluorescent group-fueled chain releasing from Fe3O4@C nanoparticles with enhanced fluorescence response. The designed platform exhibits highly sensitive in a wide linear concentration range of 0.450 pM–190 pM and is highly specific for miRNA-20a detection with the ability of discriminating one mistake base. Additionally, the CHA-Fe3O4@C was successfully applied in imaging visualization of miRNA-20a in the living cell. The strategy provides a promising bioassay approach for clinical research.
KW - Catalytic hairpin assembly
KW - FeO@C nanoparticle
KW - Intracellular visualization detection
KW - Signal amplification
KW - miRNA
UR - https://www.scopus.com/pages/publications/85092104837
U2 - 10.1016/j.talanta.2020.121675
DO - 10.1016/j.talanta.2020.121675
M3 - 文章
C2 - 33303136
AN - SCOPUS:85092104837
SN - 0039-9140
VL - 223
JO - Talanta
JF - Talanta
M1 - 121675
ER -