@inbook{d3363363caca4811b00917b2877a0fed,
title = "Creation of antigen-dependent β-lactamase fusion protein tethered by circularly permuted antibody variable domains",
abstract = "Antibody-based molecular switches that are able to recognize a range of exogenous antigens can be highly useful as a versatile biosensor. However, regulating the catalytic activity of enzymes by antibodies is still hard to achieve. Here, we describe a design method of unique antibody variable region Fv introduced with two circular permutations, called Clampbody. By tethering the Clampbody to a circularly permuted TEM-1 β-lactamase (BLA), we successfully constructed a genetically encoded molecular switch Cbody-cpBLA that shows antigen-dependent catalytic activity.",
keywords = "Allosteric regulation, Antibody variable region, Circular permutation, Immunosensor, TEM-1 β-lactamase",
author = "Hiroto Iwai and Miki Kojima-Misaizu and Jinhua Dong and Hiroshi Ueda",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media LLC 2017.",
year = "2017",
doi = "10.1007/978-1-4939-6940-1\_10",
language = "英语",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "149--165",
booktitle = "Methods in Molecular Biology",
}