D-Galactose toxicity in mice is associated with mitochondrial dysfunction: Protecting effects of mitochondrial nutrient R-alpha-lipoic acid

Jiangang Long; Xuemin Wang; Hongxiang Gao; Zhi Liu; Changsheng Liu; Mingyong Miao; Xu Cui; Lester Packer; Jiankang Liu

doi:10.1007/s10522-007-9081-y

D-Galactose toxicity in mice is associated with mitochondrial dysfunction: Protecting effects of mitochondrial nutrient R-alpha-lipoic acid

Jiangang Long
, Xuemin Wang
, Hongxiang Gao
, Zhi Liu
, Changsheng Liu
, Mingyong Miao
, Xu Cui
, Lester Packer
, Jiankang Liu

Research output: Contribution to journal › Article › peer-review

72 Scopus citations

Abstract

D-Galactose (D-gal)-induced aging models in Drosophila, houseflies, mice and rats have been widely used; however, the underlying mechanisms are poorly understood. To investigate the involvement of mitochondrial dysfunction of d-gal, mitochondrial function was examined in the brain and liver of C57BL/6J mice, subjected to a treatment of D-gal with or without a concomitant treatment with a mitochondrial nutrient, R-alpha-lipoic acid (LA). D-Gal treatment induced a significant decrease in succinate-linked respiratory control ratio (RCR) and ADP/O ratio in the liver and brain, and also a significant increase in the maximum velocity (Vmax) and substrate binding affinity (Km) of complex II in the liver. LA treatment to D-gal-injected animals restored mitochondrial RCR in both brain and liver, ADP/O and Km of complex II in the liver. These results suggest LA is effective in delaying D-gal toxicity by ameliorating mitochondrial dysfunction.

Original language	English
Pages (from-to)	373-381
Number of pages	9
Journal	Biogerontology
Volume	8
Issue number	3
DOIs	https://doi.org/10.1007/s10522-007-9081-y
State	Published - Jun 2007
Externally published	Yes

Keywords

Aging
D-Galactose
Mitochondrial dysfunction
R-alpha-Lipoic acid
Succinate-linked respiratory chain

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10.1007/s10522-007-9081-y

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title = "D-Galactose toxicity in mice is associated with mitochondrial dysfunction: Protecting effects of mitochondrial nutrient R-alpha-lipoic acid",

abstract = "D-Galactose (D-gal)-induced aging models in Drosophila, houseflies, mice and rats have been widely used; however, the underlying mechanisms are poorly understood. To investigate the involvement of mitochondrial dysfunction of d-gal, mitochondrial function was examined in the brain and liver of C57BL/6J mice, subjected to a treatment of D-gal with or without a concomitant treatment with a mitochondrial nutrient, R-alpha-lipoic acid (LA). D-Gal treatment induced a significant decrease in succinate-linked respiratory control ratio (RCR) and ADP/O ratio in the liver and brain, and also a significant increase in the maximum velocity (Vmax) and substrate binding affinity (Km) of complex II in the liver. LA treatment to D-gal-injected animals restored mitochondrial RCR in both brain and liver, ADP/O and Km of complex II in the liver. These results suggest LA is effective in delaying D-gal toxicity by ameliorating mitochondrial dysfunction.",

keywords = "Aging, D-Galactose, Mitochondrial dysfunction, R-alpha-Lipoic acid, Succinate-linked respiratory chain",

author = "Jiangang Long and Xuemin Wang and Hongxiang Gao and Zhi Liu and Changsheng Liu and Mingyong Miao and Xu Cui and Lester Packer and Jiankang Liu",

year = "2007",

month = jun,

doi = "10.1007/s10522-007-9081-y",

language = "英语",

volume = "8",

pages = "373--381",

journal = "Biogerontology",

issn = "1389-5729",

publisher = "Springer Science and Business Media B.V.",

number = "3",

}

TY - JOUR

T1 - D-Galactose toxicity in mice is associated with mitochondrial dysfunction

T2 - Protecting effects of mitochondrial nutrient R-alpha-lipoic acid

AU - Long, Jiangang

AU - Wang, Xuemin

AU - Gao, Hongxiang

AU - Liu, Zhi

AU - Liu, Changsheng

AU - Miao, Mingyong

AU - Cui, Xu

AU - Packer, Lester

AU - Liu, Jiankang

PY - 2007/6

Y1 - 2007/6

N2 - D-Galactose (D-gal)-induced aging models in Drosophila, houseflies, mice and rats have been widely used; however, the underlying mechanisms are poorly understood. To investigate the involvement of mitochondrial dysfunction of d-gal, mitochondrial function was examined in the brain and liver of C57BL/6J mice, subjected to a treatment of D-gal with or without a concomitant treatment with a mitochondrial nutrient, R-alpha-lipoic acid (LA). D-Gal treatment induced a significant decrease in succinate-linked respiratory control ratio (RCR) and ADP/O ratio in the liver and brain, and also a significant increase in the maximum velocity (Vmax) and substrate binding affinity (Km) of complex II in the liver. LA treatment to D-gal-injected animals restored mitochondrial RCR in both brain and liver, ADP/O and Km of complex II in the liver. These results suggest LA is effective in delaying D-gal toxicity by ameliorating mitochondrial dysfunction.

AB - D-Galactose (D-gal)-induced aging models in Drosophila, houseflies, mice and rats have been widely used; however, the underlying mechanisms are poorly understood. To investigate the involvement of mitochondrial dysfunction of d-gal, mitochondrial function was examined in the brain and liver of C57BL/6J mice, subjected to a treatment of D-gal with or without a concomitant treatment with a mitochondrial nutrient, R-alpha-lipoic acid (LA). D-Gal treatment induced a significant decrease in succinate-linked respiratory control ratio (RCR) and ADP/O ratio in the liver and brain, and also a significant increase in the maximum velocity (Vmax) and substrate binding affinity (Km) of complex II in the liver. LA treatment to D-gal-injected animals restored mitochondrial RCR in both brain and liver, ADP/O and Km of complex II in the liver. These results suggest LA is effective in delaying D-gal toxicity by ameliorating mitochondrial dysfunction.

KW - Aging

KW - D-Galactose

KW - Mitochondrial dysfunction

KW - R-alpha-Lipoic acid

KW - Succinate-linked respiratory chain

UR - https://www.scopus.com/pages/publications/34248159506

U2 - 10.1007/s10522-007-9081-y

DO - 10.1007/s10522-007-9081-y

M3 - 文章

C2 - 17473993

AN - SCOPUS:34248159506

SN - 1389-5729

VL - 8

SP - 373

EP - 381

JO - Biogerontology

JF - Biogerontology

IS - 3

ER -

D-Galactose toxicity in mice is associated with mitochondrial dysfunction: Protecting effects of mitochondrial nutrient R-alpha-lipoic acid

Abstract

Keywords

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