Deoxyribonuclease 1-like 3 may be a Potential Prognostic Biomarker Associated with Immune Infiltration in Colon Cancer

Jing Liu; Jingya Yi; Zhihong Zhang; Donglin Cao; Lei Li; Yachao Yao

doi:10.18632/aging.203173

Deoxyribonuclease 1-like 3 may be a Potential Prognostic Biomarker Associated with Immune Infiltration in Colon Cancer

Jing Liu
, Jingya Yi
, Zhihong Zhang
, Donglin Cao
, Lei Li
, Yachao Yao

Guangdong Second Provincial General Hospital
Southern Medical University
Guangzhou Medical College
Key Laboratory for Reproductive Medicine of Guangdong Province

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Colon adenocarcinoma (COAD) is a common cancer of the digestive system. It’s high morbidity and mortality make it one of the leading causes of cancer deaths. In this study, we studied the microenvironment of colon cancer to find new diagnostic markers and immunotherapy targets for colon cancer. Tumor purity of colon cancer samples in TCGA database were obtained by ESTIMATE algorithm. Then, we analyzed the association of Immune, Stromal, and Estimate scores with tumor prognosis and clinicopathological features. By comparing the gene expression profiles between tumor and normal samples, the high and low immune score groups, 117 intersecting differentially expressed genes (DEGs) were obtained. The function, molecular pathway, and prognostic value of these 117 DEGs pointed toward the importance of deoxyribonuclease 1-like 3 (DNASE1L3). Validation results from multiple databases showed low expression of DNASE1L3 in colon cancer. A single GSEA and correlation analysis of immune cells indicated that DNASE1L3 was closely related to immunity. The low expression of DNASE1L3 in colon cancer samples was measured with qRT-PCR. The scratch and cell proliferation experimentssuggested that DNASE1L3 may affect cell migration. Therefore, we concluded that DNASE1L3 might be a biomarker associated with prognosis and immune infiltration in colon cancer.

Original language	English
Pages (from-to)	16513-16526
Number of pages	14
Journal	Aging
Volume	13
Issue number	12
DOIs	https://doi.org/10.18632/aging.203173
State	Published - 30 Jun 2021
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

The Cancer Genome Atlas (TCGA) Database
colon adenocarcinoma
deoxyribonuclease 1-like 3
immune infiltration
tumor microenvironment

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10.18632/aging.203173

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title = "Deoxyribonuclease 1-like 3 may be a Potential Prognostic Biomarker Associated with Immune Infiltration in Colon Cancer",

abstract = "Colon adenocarcinoma (COAD) is a common cancer of the digestive system. It{\textquoteright}s high morbidity and mortality make it one of the leading causes of cancer deaths. In this study, we studied the microenvironment of colon cancer to find new diagnostic markers and immunotherapy targets for colon cancer. Tumor purity of colon cancer samples in TCGA database were obtained by ESTIMATE algorithm. Then, we analyzed the association of Immune, Stromal, and Estimate scores with tumor prognosis and clinicopathological features. By comparing the gene expression profiles between tumor and normal samples, the high and low immune score groups, 117 intersecting differentially expressed genes (DEGs) were obtained. The function, molecular pathway, and prognostic value of these 117 DEGs pointed toward the importance of deoxyribonuclease 1-like 3 (DNASE1L3). Validation results from multiple databases showed low expression of DNASE1L3 in colon cancer. A single GSEA and correlation analysis of immune cells indicated that DNASE1L3 was closely related to immunity. The low expression of DNASE1L3 in colon cancer samples was measured with qRT-PCR. The scratch and cell proliferation experimentssuggested that DNASE1L3 may affect cell migration. Therefore, we concluded that DNASE1L3 might be a biomarker associated with prognosis and immune infiltration in colon cancer.",

keywords = "The Cancer Genome Atlas (TCGA) Database, colon adenocarcinoma, deoxyribonuclease 1-like 3, immune infiltration, tumor microenvironment",

author = "Jing Liu and Jingya Yi and Zhihong Zhang and Donglin Cao and Lei Li and Yachao Yao",

note = "Publisher Copyright: {\textcopyright} 2021 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2021",

month = jun,

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T1 - Deoxyribonuclease 1-like 3 may be a Potential Prognostic Biomarker Associated with Immune Infiltration in Colon Cancer

AU - Liu, Jing

AU - Yi, Jingya

AU - Zhang, Zhihong

AU - Cao, Donglin

AU - Li, Lei

AU - Yao, Yachao

N1 - Publisher Copyright: © 2021 Liu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2021/6/30

Y1 - 2021/6/30

N2 - Colon adenocarcinoma (COAD) is a common cancer of the digestive system. It’s high morbidity and mortality make it one of the leading causes of cancer deaths. In this study, we studied the microenvironment of colon cancer to find new diagnostic markers and immunotherapy targets for colon cancer. Tumor purity of colon cancer samples in TCGA database were obtained by ESTIMATE algorithm. Then, we analyzed the association of Immune, Stromal, and Estimate scores with tumor prognosis and clinicopathological features. By comparing the gene expression profiles between tumor and normal samples, the high and low immune score groups, 117 intersecting differentially expressed genes (DEGs) were obtained. The function, molecular pathway, and prognostic value of these 117 DEGs pointed toward the importance of deoxyribonuclease 1-like 3 (DNASE1L3). Validation results from multiple databases showed low expression of DNASE1L3 in colon cancer. A single GSEA and correlation analysis of immune cells indicated that DNASE1L3 was closely related to immunity. The low expression of DNASE1L3 in colon cancer samples was measured with qRT-PCR. The scratch and cell proliferation experimentssuggested that DNASE1L3 may affect cell migration. Therefore, we concluded that DNASE1L3 might be a biomarker associated with prognosis and immune infiltration in colon cancer.

AB - Colon adenocarcinoma (COAD) is a common cancer of the digestive system. It’s high morbidity and mortality make it one of the leading causes of cancer deaths. In this study, we studied the microenvironment of colon cancer to find new diagnostic markers and immunotherapy targets for colon cancer. Tumor purity of colon cancer samples in TCGA database were obtained by ESTIMATE algorithm. Then, we analyzed the association of Immune, Stromal, and Estimate scores with tumor prognosis and clinicopathological features. By comparing the gene expression profiles between tumor and normal samples, the high and low immune score groups, 117 intersecting differentially expressed genes (DEGs) were obtained. The function, molecular pathway, and prognostic value of these 117 DEGs pointed toward the importance of deoxyribonuclease 1-like 3 (DNASE1L3). Validation results from multiple databases showed low expression of DNASE1L3 in colon cancer. A single GSEA and correlation analysis of immune cells indicated that DNASE1L3 was closely related to immunity. The low expression of DNASE1L3 in colon cancer samples was measured with qRT-PCR. The scratch and cell proliferation experimentssuggested that DNASE1L3 may affect cell migration. Therefore, we concluded that DNASE1L3 might be a biomarker associated with prognosis and immune infiltration in colon cancer.

KW - The Cancer Genome Atlas (TCGA) Database

KW - colon adenocarcinoma

KW - deoxyribonuclease 1-like 3

KW - immune infiltration

KW - tumor microenvironment

UR - https://www.scopus.com/pages/publications/85109157551

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M3 - 文章

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AN - SCOPUS:85109157551

SN - 1945-4589

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EP - 16526

JO - Aging

JF - Aging

IS - 12

ER -

Deoxyribonuclease 1-like 3 may be a Potential Prognostic Biomarker Associated with Immune Infiltration in Colon Cancer

Abstract

UN SDGs

Keywords

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