Effect of GABA                         B                          receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats

Cai Feng Shao; Wen Wei Zhang; Chao Ting Hu; Ran Chao Wang; Yu Jie; Chang Yun Zhao; Qian Chen; Kun Yang

doi:10.3969/j.issn.1001-1978.2018.11.008

Effect of GABA _B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats

Cai Feng Shao
, Wen Wei Zhang
, Chao Ting Hu
, Ran Chao Wang
, Yu Jie
, Chang Yun Zhao
, Qian Chen
, Kun Yang

Jiangsu University
Affiliated People's Hospital of Jiangsu University

Research output: Contribution to journal › Article › peer-review

Abstract

Aim: To investigate the possible difference between glutamatergic synapses and GABAergic synapses in the ventrolateral periaqueductal gray (vlPAG) when GABA _B receptor agonist is at nonsaturating concentration or saturating concentration. Methods We employed whole cell patch-clamp recordings on acute PAG slices from adult rats. Results Glutamatergic synapses were less sensitive to baclofen, a putative GABA _B receptor agonist, than that of GABAergic synapses, but only at nonsaturating concentration (0.1 μmol • L ^-1 ). On the contrary, saturating concentration of baclofen (5 μmol • L ^-1 ) inhibited both glutamatergic and GABAergic synapses to a similar ratio. Accordingly, low concentration of baclofen increased, while high concentration decreased the excitability of postsynaptic vlPAG neurons. Conclusions The overall action of baclofen in vlPAG depends on its concentrations. Since vlPAG plays an important role in reducing pain modulation system, the different concentrations of GABA may induce its varying actions on modulation.

Original language	English
Pages (from-to)	1511-1515
Number of pages	5
Journal	Chinese Pharmacological Bulletin
Volume	34
Issue number	11
DOIs	https://doi.org/10.3969/j.issn.1001-1978.2018.11.008
State	Published - Nov 2018
Externally published	Yes

Keywords

Excitatory synapses
GABA Receptors
Inhibitory synapses
PAG
Rat
Whole-cell recordings

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10.3969/j.issn.1001-1978.2018.11.008

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Shao, C. F., Zhang, W. W., Hu, C. T., Wang, R. C., Jie, Y., Zhao, C. Y., Chen, Q., & Yang, K. (2018). Effect of GABA _B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats Chinese Pharmacological Bulletin, 34(11), 1511-1515. https://doi.org/10.3969/j.issn.1001-1978.2018.11.008

@article{4b29afe6f8954cc6a409de14e59b7420,

title = " Effect of GABA B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats ",

abstract = " Aim: To investigate the possible difference between glutamatergic synapses and GABAergic synapses in the ventrolateral periaqueductal gray (vlPAG) when GABA B receptor agonist is at nonsaturating concentration or saturating concentration. Methods We employed whole cell patch-clamp recordings on acute PAG slices from adult rats. Results Glutamatergic synapses were less sensitive to baclofen, a putative GABA B receptor agonist, than that of GABAergic synapses, but only at nonsaturating concentration (0.1 μmol • L -1 ). On the contrary, saturating concentration of baclofen (5 μmol • L -1 ) inhibited both glutamatergic and GABAergic synapses to a similar ratio. Accordingly, low concentration of baclofen increased, while high concentration decreased the excitability of postsynaptic vlPAG neurons. Conclusions The overall action of baclofen in vlPAG depends on its concentrations. Since vlPAG plays an important role in reducing pain modulation system, the different concentrations of GABA may induce its varying actions on modulation.",

keywords = "Excitatory synapses, GABA Receptors, Inhibitory synapses, PAG, Rat, Whole-cell recordings",

author = "Shao, \{Cai Feng\} and Zhang, \{Wen Wei\} and Hu, \{Chao Ting\} and Wang, \{Ran Chao\} and Yu Jie and Zhao, \{Chang Yun\} and Qian Chen and Kun Yang",

year = "2018",

month = nov,

doi = "10.3969/j.issn.1001-1978.2018.11.008",

language = "英语",

volume = "34",

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journal = "Chinese Pharmacological Bulletin",

issn = "1001-1978",

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number = "11",

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Shao, CF, Zhang, WW, Hu, CT, Wang, RC, Jie, Y, Zhao, CY, Chen, Q & Yang, K 2018, ' Effect of GABA _B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats ', Chinese Pharmacological Bulletin, vol. 34, no. 11, pp. 1511-1515. https://doi.org/10.3969/j.issn.1001-1978.2018.11.008

Effect of GABA _B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats . / Shao, Cai Feng; Zhang, Wen Wei; Hu, Chao Ting et al.
In: Chinese Pharmacological Bulletin, Vol. 34, No. 11, 11.2018, p. 1511-1515.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Effect of GABA B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats

AU - Shao, Cai Feng

AU - Zhang, Wen Wei

AU - Hu, Chao Ting

AU - Wang, Ran Chao

AU - Jie, Yu

AU - Zhao, Chang Yun

AU - Chen, Qian

AU - Yang, Kun

PY - 2018/11

Y1 - 2018/11

N2 - Aim: To investigate the possible difference between glutamatergic synapses and GABAergic synapses in the ventrolateral periaqueductal gray (vlPAG) when GABA B receptor agonist is at nonsaturating concentration or saturating concentration. Methods We employed whole cell patch-clamp recordings on acute PAG slices from adult rats. Results Glutamatergic synapses were less sensitive to baclofen, a putative GABA B receptor agonist, than that of GABAergic synapses, but only at nonsaturating concentration (0.1 μmol • L -1 ). On the contrary, saturating concentration of baclofen (5 μmol • L -1 ) inhibited both glutamatergic and GABAergic synapses to a similar ratio. Accordingly, low concentration of baclofen increased, while high concentration decreased the excitability of postsynaptic vlPAG neurons. Conclusions The overall action of baclofen in vlPAG depends on its concentrations. Since vlPAG plays an important role in reducing pain modulation system, the different concentrations of GABA may induce its varying actions on modulation.

AB - Aim: To investigate the possible difference between glutamatergic synapses and GABAergic synapses in the ventrolateral periaqueductal gray (vlPAG) when GABA B receptor agonist is at nonsaturating concentration or saturating concentration. Methods We employed whole cell patch-clamp recordings on acute PAG slices from adult rats. Results Glutamatergic synapses were less sensitive to baclofen, a putative GABA B receptor agonist, than that of GABAergic synapses, but only at nonsaturating concentration (0.1 μmol • L -1 ). On the contrary, saturating concentration of baclofen (5 μmol • L -1 ) inhibited both glutamatergic and GABAergic synapses to a similar ratio. Accordingly, low concentration of baclofen increased, while high concentration decreased the excitability of postsynaptic vlPAG neurons. Conclusions The overall action of baclofen in vlPAG depends on its concentrations. Since vlPAG plays an important role in reducing pain modulation system, the different concentrations of GABA may induce its varying actions on modulation.

KW - Excitatory synapses

KW - GABA Receptors

KW - Inhibitory synapses

KW - PAG

KW - Rat

KW - Whole-cell recordings

UR - https://www.scopus.com/pages/publications/85059666512

U2 - 10.3969/j.issn.1001-1978.2018.11.008

DO - 10.3969/j.issn.1001-1978.2018.11.008

M3 - 文章

AN - SCOPUS:85059666512

SN - 1001-1978

VL - 34

SP - 1511

EP - 1515

JO - Chinese Pharmacological Bulletin

JF - Chinese Pharmacological Bulletin

IS - 11

ER -

Shao CF, Zhang WW, Hu CT, Wang RC, Jie Y, Zhao CY et al. Effect of GABA _B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats Chinese Pharmacological Bulletin. 2018 Nov;34(11):1511-1515. doi: 10.3969/j.issn.1001-1978.2018.11.008

Effect of GABA _B receptor agonist baclofen on excitatory and inhibitory synapses of ventrolateral periaqueductal gray in rats

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