Effects of combined use of heparinoid, aspirin, and losartan on the experimental vascular intima hyperplasia in rats

AIM: To observe the effects of the combination of heparinoid, aspirin and losartan on the experimental vascular intima hyperplasia induced by squeezing carotid artery in rats. METHODS: Rats except the normal control group were under operation after two days oral administration once a day. At the second hour after injury, blood coagulation time(CT), time of arterial thrombus formation by electrical stimulus (ATFTES) and the level of TXB₂ in plasma were measured. At the fourteenth day after the surgery, the following indexes were examined: intima/media ratio, intima area ratio, PDGF-B and PCNA immunohistochemistry in carotid artery. RESULTS: CT, TATFES increased and the level of TXB₂ decreased in the groups of Hep, Asp and Hep+Asp. The PDGF-B content, the positive ratio of PCNA and the intima/media ratio also decreased in the groups of Hep, Asp, Los, Hep+Asp, Hep+Los and Asp+ Los. Compared with single drug or two drugs combination, PDGF-B, positive ratio of PCNA, intima/media ratio and intima area ratio decreased significantly in the group of three drugs combination (P < 0. 01), while the effects on CT, TATFES and level of TXB₂ were not significant. CONCLUSION: The combination of heperinoid, aspirin and losartan had a complementary and cooperative effect on inhibiting the experimental vascular intima hyperplasia.