Luciferase Complementation Imaging Assay (LCI) to Reveal the Interaction Between SPL7 and RAN1 Genes During Ethylene Signaling

Yanzhi Yang; Lei Li

doi:10.1007/978-1-0716-4650-2_14

Luciferase Complementation Imaging Assay (LCI) to Reveal the Interaction Between SPL7 and RAN1 Genes During Ethylene Signaling

Yanzhi Yang
, Lei Li

Peking University

Research output: Chapter in Book/Report/Conference proceeding › Chapter › peer-review

1 Scopus citations

Abstract

The gaseous hormone ethylene regulates multiple aspects of plant growth and development, as well as responses to biotic and abiotic factors. The copper transporter RAN1 mediates the delivery of copper ions to ETR1 and is crucial for proper ethylene receptor function and thus proper ethylene signaling. Moreover, the central regulator of copper homeostasis, SPL7 interacts with RAN1 at the endoplasmic reticulum to modulate ethylene signaling. Here, we present a detailed protocol of luciferase complementation imaging (LCI), a bioluminescence technique, to examine the interaction between membrane-bound proteins, such as SPL7 and RAN1, in ethylene signaling.

Original language	English
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	193-201
Number of pages	9
DOIs	https://doi.org/10.1007/978-1-0716-4650-2_14
State	Published - 2025
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	2945
ISSN (Print)	1064-3745
ISSN (Electronic)	1940-6029

Keywords

Agrobacterium tumefaciens
Ethylene
Interaction
Luciferase complementation imaging
Nicotiana benthamiana

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10.1007/978-1-0716-4650-2_14

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title = "Luciferase Complementation Imaging Assay (LCI) to Reveal the Interaction Between SPL7 and RAN1 Genes During Ethylene Signaling",

abstract = "The gaseous hormone ethylene regulates multiple aspects of plant growth and development, as well as responses to biotic and abiotic factors. The copper transporter RAN1 mediates the delivery of copper ions to ETR1 and is crucial for proper ethylene receptor function and thus proper ethylene signaling. Moreover, the central regulator of copper homeostasis, SPL7 interacts with RAN1 at the endoplasmic reticulum to modulate ethylene signaling. Here, we present a detailed protocol of luciferase complementation imaging (LCI), a bioluminescence technique, to examine the interaction between membrane-bound proteins, such as SPL7 and RAN1, in ethylene signaling.",

keywords = "Agrobacterium tumefaciens, Ethylene, Interaction, Luciferase complementation imaging, Nicotiana benthamiana",

author = "Yanzhi Yang and Lei Li",

note = "Publisher Copyright: {\textcopyright} The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.",

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doi = "10.1007/978-1-0716-4650-2\_14",

language = "英语",

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AU - Yang, Yanzhi

AU - Li, Lei

N1 - Publisher Copyright: © The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2025.

PY - 2025

Y1 - 2025

N2 - The gaseous hormone ethylene regulates multiple aspects of plant growth and development, as well as responses to biotic and abiotic factors. The copper transporter RAN1 mediates the delivery of copper ions to ETR1 and is crucial for proper ethylene receptor function and thus proper ethylene signaling. Moreover, the central regulator of copper homeostasis, SPL7 interacts with RAN1 at the endoplasmic reticulum to modulate ethylene signaling. Here, we present a detailed protocol of luciferase complementation imaging (LCI), a bioluminescence technique, to examine the interaction between membrane-bound proteins, such as SPL7 and RAN1, in ethylene signaling.

AB - The gaseous hormone ethylene regulates multiple aspects of plant growth and development, as well as responses to biotic and abiotic factors. The copper transporter RAN1 mediates the delivery of copper ions to ETR1 and is crucial for proper ethylene receptor function and thus proper ethylene signaling. Moreover, the central regulator of copper homeostasis, SPL7 interacts with RAN1 at the endoplasmic reticulum to modulate ethylene signaling. Here, we present a detailed protocol of luciferase complementation imaging (LCI), a bioluminescence technique, to examine the interaction between membrane-bound proteins, such as SPL7 and RAN1, in ethylene signaling.

KW - Agrobacterium tumefaciens

KW - Ethylene

KW - Interaction

KW - Luciferase complementation imaging

KW - Nicotiana benthamiana

UR - https://www.scopus.com/pages/publications/105017691480

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DO - 10.1007/978-1-0716-4650-2_14

M3 - 章节

C2 - 41028706

AN - SCOPUS:105017691480

T3 - Methods in Molecular Biology

SP - 193

EP - 201

BT - Methods in Molecular Biology

PB - Humana Press Inc.

ER -

Luciferase Complementation Imaging Assay (LCI) to Reveal the Interaction Between SPL7 and RAN1 Genes During Ethylene Signaling

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