Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar

Yayu Guo; Shufang Wang; Keji Yu; Hou Ling Wang; Huimin Xu; Chengwei Song; Yuanyuan Zhao; Jialong Wen; Chunxiang Fu; Yu Li; Shuizhong Wang; Xi Zhang; Yan Zhang; Yuan Cao; Fenjuan Shao; Xiaohua Wang; Xin Deng; Tong Chen; Qiao Zhao; Lei Li; Guodong Wang; Paul Grünhofer; Lukas Schreiber; Yue Li; Guoyong Song; Richard A. Dixon; Jinxing Lin

doi:10.1038/s41467-023-39930-3

Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar

Yayu Guo
, Shufang Wang
, Keji Yu
, Hou Ling Wang
, Huimin Xu
, Chengwei Song
, Yuanyuan Zhao
, Jialong Wen
, Chunxiang Fu
, Yu Li
, Shuizhong Wang
, Xi Zhang
, Yan Zhang
, Yuan Cao
, Fenjuan Shao
, Xiaohua Wang
, Xin Deng
, Tong Chen
, Qiao Zhao
, Lei Li

Guodong Wang, Paul Grünhofer, Lukas Schreiber, Yue Li, Guoyong Song, Richard A. Dixon, Jinxing Lin

Beijing Forestry University
Institute of Botany, Chinese Academy of Sciences
China Agricultural University
Henan University of Science and Technology
CAS - Qingdao Institute of Biomass Energy and Bioprocess Technology
Chinese Academy of Forestry
Shenzhen Institute of Advanced Technology
Peking University
CAS - Institute of Genetics and Developmental Biology
University of Bonn
University of North Texas

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.

Original language	English
Article number	4285
Journal	Nature Communications
Volume	14
Issue number	1
DOIs	https://doi.org/10.1038/s41467-023-39930-3
State	Published - Dec 2023
Externally published	Yes

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Guo, Y., Wang, S., Yu, K., Wang, H. L., Xu, H., Song, C., Zhao, Y., Wen, J., Fu, C., Li, Y., Wang, S., Zhang, X., Zhang, Y., Cao, Y., Shao, F., Wang, X., Deng, X., Chen, T., Zhao, Q., ... Lin, J. (2023). Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar. Nature Communications, 14(1), Article 4285. https://doi.org/10.1038/s41467-023-39930-3

@article{37aa41b3c4ab44c29a687b21fe9403df,

title = "Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar",

abstract = "The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.",

author = "Yayu Guo and Shufang Wang and Keji Yu and Wang, \{Hou Ling\} and Huimin Xu and Chengwei Song and Yuanyuan Zhao and Jialong Wen and Chunxiang Fu and Yu Li and Shuizhong Wang and Xi Zhang and Yan Zhang and Yuan Cao and Fenjuan Shao and Xiaohua Wang and Xin Deng and Tong Chen and Qiao Zhao and Lei Li and Guodong Wang and Paul Gr{\"u}nhofer and Lukas Schreiber and Yue Li and Guoyong Song and Dixon, \{Richard A.\} and Jinxing Lin",

note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",

year = "2023",

month = dec,

doi = "10.1038/s41467-023-39930-3",

language = "英语",

volume = "14",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

number = "1",

}

Guo, Y, Wang, S, Yu, K, Wang, HL, Xu, H, Song, C, Zhao, Y, Wen, J, Fu, C, Li, Y, Wang, S, Zhang, X, Zhang, Y, Cao, Y, Shao, F, Wang, X, Deng, X, Chen, T, Zhao, Q, Li, L, Wang, G, Grünhofer, P, Schreiber, L, Li, Y, Song, G, Dixon, RA & Lin, J 2023, 'Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar', Nature Communications, vol. 14, no. 1, 4285. https://doi.org/10.1038/s41467-023-39930-3

TY - JOUR

T1 - Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar

AU - Guo, Yayu

AU - Wang, Shufang

AU - Yu, Keji

AU - Wang, Hou Ling

AU - Xu, Huimin

AU - Song, Chengwei

AU - Zhao, Yuanyuan

AU - Wen, Jialong

AU - Fu, Chunxiang

AU - Li, Yu

AU - Wang, Shuizhong

AU - Zhang, Xi

AU - Zhang, Yan

AU - Cao, Yuan

AU - Shao, Fenjuan

AU - Wang, Xiaohua

AU - Deng, Xin

AU - Chen, Tong

AU - Zhao, Qiao

AU - Li, Lei

AU - Wang, Guodong

AU - Grünhofer, Paul

AU - Schreiber, Lukas

AU - Li, Yue

AU - Song, Guoyong

AU - Dixon, Richard A.

AU - Lin, Jinxing

PY - 2023/12

Y1 - 2023/12

N2 - The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.

AB - The conversion of lignocellulosic feedstocks to fermentable sugar for biofuel production is inefficient, and most strategies to enhance efficiency directly target lignin biosynthesis, with associated negative growth impacts. Here we demonstrate, for both laboratory- and field-grown plants, that expression of Pag-miR408 in poplar (Populus alba × P. glandulosa) significantly enhances saccharification, with no requirement for acid-pretreatment, while promoting plant growth. The overexpression plants show increased accessibility of cell walls to cellulase and scaffoldin cellulose-binding modules. Conversely, Pag-miR408 loss-of-function poplar shows decreased cell wall accessibility. Overexpression of Pag-miR408 targets three Pag-LACCASES, delays lignification, and modestly reduces lignin content, S/G ratio and degree of lignin polymerization. Meanwhile, the LACCASE loss of function mutants exhibit significantly increased growth and cell wall accessibility in xylem. Our study shows how Pag-miR408 regulates lignification and secondary growth, and suggest an effective approach towards enhancing biomass yield and saccharification efficiency in a major bioenergy crop.

UR - https://www.scopus.com/pages/publications/85165260825

U2 - 10.1038/s41467-023-39930-3

DO - 10.1038/s41467-023-39930-3

M3 - 文章

C2 - 37463897

AN - SCOPUS:85165260825

SN - 2041-1723

VL - 14

JO - Nature Communications

JF - Nature Communications

IS - 1

M1 - 4285

ER -

Manipulating microRNA miR408 enhances both biomass yield and saccharification efficiency in poplar

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