Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level

Fengjiao Zhu; Yahui Ji; Jiu Deng; Linmei Li; Xue Bai; Xianming Liu; Bingcheng Lin; Yao Lu

doi:10.1016/j.cclet.2021.09.058

Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level

Fengjiao Zhu
, Yahui Ji
, Jiu Deng
, Linmei Li
, Xue Bai
, Xianming Liu
, Bingcheng Lin
, Yao Lu

Dalian Institute of Chemical Physics Chinese Academy of Sciences
University of Chinese Academy of Sciences

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Extracellular vesicles (EVs) are membrane vesicles secreted by cells, playing critical roles in mediating intercellular communications for various physiological and pathological processes. Most of the EV analysis is currently performed at the bulk level, obscuring the origin of the EVs and diverse characteristics of the individual extracellular vesicle. Technologies to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV secretion. Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims. Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis, which would be summarized in this mini-review.

Original language	English
Pages (from-to)	2893-2900
Number of pages	8
Journal	Chinese Chemical Letters
Volume	33
Issue number	6
DOIs	https://doi.org/10.1016/j.cclet.2021.09.058
State	Published - Jun 2022
Externally published	Yes

Keywords

Extracellular vesicle
Heterogeneity
Microfluidics
Single EV analysis
Single-cell EV analysis

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10.1016/j.cclet.2021.09.058

Cite this

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title = "Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level",

abstract = "Extracellular vesicles (EVs) are membrane vesicles secreted by cells, playing critical roles in mediating intercellular communications for various physiological and pathological processes. Most of the EV analysis is currently performed at the bulk level, obscuring the origin of the EVs and diverse characteristics of the individual extracellular vesicle. Technologies to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV secretion. Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims. Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis, which would be summarized in this mini-review.",

keywords = "Extracellular vesicle, Heterogeneity, Microfluidics, Single EV analysis, Single-cell EV analysis",

author = "Fengjiao Zhu and Yahui Ji and Jiu Deng and Linmei Li and Xue Bai and Xianming Liu and Bingcheng Lin and Yao Lu",

note = "Publisher Copyright: {\textcopyright} 2021",

year = "2022",

month = jun,

doi = "10.1016/j.cclet.2021.09.058",

language = "英语",

volume = "33",

pages = "2893--2900",

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TY - JOUR

T1 - Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level

AU - Zhu, Fengjiao

AU - Ji, Yahui

AU - Deng, Jiu

AU - Li, Linmei

AU - Bai, Xue

AU - Liu, Xianming

AU - Lin, Bingcheng

AU - Lu, Yao

PY - 2022/6

Y1 - 2022/6

N2 - Extracellular vesicles (EVs) are membrane vesicles secreted by cells, playing critical roles in mediating intercellular communications for various physiological and pathological processes. Most of the EV analysis is currently performed at the bulk level, obscuring the origin of the EVs and diverse characteristics of the individual extracellular vesicle. Technologies to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV secretion. Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims. Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis, which would be summarized in this mini-review.

AB - Extracellular vesicles (EVs) are membrane vesicles secreted by cells, playing critical roles in mediating intercellular communications for various physiological and pathological processes. Most of the EV analysis is currently performed at the bulk level, obscuring the origin of the EVs and diverse characteristics of the individual extracellular vesicle. Technologies to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV secretion. Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims. Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis, which would be summarized in this mini-review.

KW - Extracellular vesicle

KW - Heterogeneity

KW - Microfluidics

KW - Single EV analysis

KW - Single-cell EV analysis

UR - https://www.scopus.com/pages/publications/85120741167

U2 - 10.1016/j.cclet.2021.09.058

DO - 10.1016/j.cclet.2021.09.058

M3 - 文章

AN - SCOPUS:85120741167

SN - 1001-8417

VL - 33

SP - 2893

EP - 2900

JO - Chinese Chemical Letters

JF - Chinese Chemical Letters

IS - 6

ER -

Microfluidics-based technologies for the analysis of extracellular vesicles at the single-cell level and single-vesicle level

Abstract

Keywords

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