Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells

Nemanja Vukašinović; Che Wei Hsu; Marco Marconi; Shaopeng Li; Christopher Zachary; Rachel Shahan; Pablo Szekley; Ziv Aardening; Isabelle Vanhoutte; Qian Ma; Lucrezia Pinto; Pavel Krupař; Nathan German; Jingyuan Zhang; Claire Simon--Vezo; Jessica Perez-Sancho; Pepe Cana Quijada; Qianzi Zhou; Laura R. Lee; Jianghua Cai; Emmanuelle M. Bayer; Matyáš Fendrych; Elisabeth Truernit; Yu Zhou; Sigal Savaldi-Goldstein; Krzysztof Wabnik; Trevor M. Nolan; Eugenia Russinova

doi:10.1016/j.cell.2025.02.011

Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells

Nemanja Vukašinović
, Che Wei Hsu
, Marco Marconi
, Shaopeng Li
, Christopher Zachary
, Rachel Shahan
, Pablo Szekley
, Ziv Aardening
, Isabelle Vanhoutte
, Qian Ma
, Lucrezia Pinto
, Pavel Krupař
, Nathan German
, Jingyuan Zhang
, Claire Simon--Vezo
, Jessica Perez-Sancho
, Pepe Cana Quijada
, Qianzi Zhou
, Laura R. Lee
, Jianghua Cai

Emmanuelle M. Bayer, Matyáš Fendrych, Elisabeth Truernit, Yu Zhou, Sigal Savaldi-Goldstein, Krzysztof Wabnik, Trevor M. Nolan, Eugenia Russinova

Ghent University
VIB-UGent Center for Plant Systems Biology
Duke University
California Institute of Technology
UPM-INIA) Universidad Politécnica de Madrid (UPM) - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA
Wuhan University
University of California at Los Angeles
Technion-Israel Institute of Technology
Charles University
Czech Academy of Sciences
Laboratoire de Biogenèse Membranaire
New York University
Chongqing University
Swiss Federal Institute of Technology Zurich
Technical University of Madrid

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis root, we reveal that brassinosteroid activity fluctuates throughout the cell cycle, decreasing during mitotic divisions and increasing during the G1 phase. The post-mitotic recovery of brassinosteroid activity is driven by the intrinsic polarity of the mother cell, resulting in one daughter cell with enhanced brassinosteroid signaling, while the other supports brassinosteroid biosynthesis. The coexistence of these distinct daughter cell states during the G1 phase circumvents a negative feedback loop to facilitate brassinosteroid production while signaling increases. Our findings uncover polarity-guided, uneven mitotic divisions in the meristem, which control brassinosteroid hormone activity to ensure optimal root growth.

Original language	English
Pages (from-to)	2063-2080.e24
Journal	Cell
Volume	188
Issue number	8
DOIs	https://doi.org/10.1016/j.cell.2025.02.011
State	Published - 17 Apr 2025
Externally published	Yes

Keywords

brassinosteroids
cell cycle
cell division
cell polarity
live-cell imaging
root meristem
single-cell RNA sequencing

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10.1016/j.cell.2025.02.011

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Vukašinović, N., Hsu, C. W., Marconi, M., Li, S., Zachary, C., Shahan, R., Szekley, P., Aardening, Z., Vanhoutte, I., Ma, Q., Pinto, L., Krupař, P., German, N., Zhang, J., Simon--Vezo, C., Perez-Sancho, J., Quijada, P. C., Zhou, Q., Lee, L. R., ... Russinova, E. (2025). Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells. Cell, 188(8), 2063-2080.e24. https://doi.org/10.1016/j.cell.2025.02.011

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title = "Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells",

abstract = "Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis root, we reveal that brassinosteroid activity fluctuates throughout the cell cycle, decreasing during mitotic divisions and increasing during the G1 phase. The post-mitotic recovery of brassinosteroid activity is driven by the intrinsic polarity of the mother cell, resulting in one daughter cell with enhanced brassinosteroid signaling, while the other supports brassinosteroid biosynthesis. The coexistence of these distinct daughter cell states during the G1 phase circumvents a negative feedback loop to facilitate brassinosteroid production while signaling increases. Our findings uncover polarity-guided, uneven mitotic divisions in the meristem, which control brassinosteroid hormone activity to ensure optimal root growth.",

keywords = "brassinosteroids, cell cycle, cell division, cell polarity, live-cell imaging, root meristem, single-cell RNA sequencing",

author = "Nemanja Vuka{\v s}inovi{\'c} and Hsu, \{Che Wei\} and Marco Marconi and Shaopeng Li and Christopher Zachary and Rachel Shahan and Pablo Szekley and Ziv Aardening and Isabelle Vanhoutte and Qian Ma and Lucrezia Pinto and Pavel Krupa{\v r} and Nathan German and Jingyuan Zhang and Claire Simon--Vezo and Jessica Perez-Sancho and Quijada, \{Pepe Cana\} and Qianzi Zhou and Lee, \{Laura R.\} and Jianghua Cai and Bayer, \{Emmanuelle M.\} and Maty{\'a}{\v s} Fendrych and Elisabeth Truernit and Yu Zhou and Sigal Savaldi-Goldstein and Krzysztof Wabnik and Nolan, \{Trevor M.\} and Eugenia Russinova",

note = "Publisher Copyright: {\textcopyright} 2025 The Authors",

year = "2025",

month = apr,

day = "17",

doi = "10.1016/j.cell.2025.02.011",

language = "英语",

volume = "188",

pages = "2063--2080.e24",

journal = "Cell",

issn = "0092-8674",

publisher = "Elsevier B.V.",

number = "8",

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Vukašinović, N, Hsu, CW, Marconi, M, Li, S, Zachary, C, Shahan, R, Szekley, P, Aardening, Z, Vanhoutte, I, Ma, Q, Pinto, L, Krupař, P, German, N, Zhang, J, Simon--Vezo, C, Perez-Sancho, J, Quijada, PC, Zhou, Q, Lee, LR, Cai, J, Bayer, EM, Fendrych, M, Truernit, E, Zhou, Y, Savaldi-Goldstein, S, Wabnik, K, Nolan, TM & Russinova, E 2025, 'Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells', Cell, vol. 188, no. 8, pp. 2063-2080.e24. https://doi.org/10.1016/j.cell.2025.02.011

TY - JOUR

T1 - Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells

AU - Vukašinović, Nemanja

AU - Hsu, Che Wei

AU - Marconi, Marco

AU - Li, Shaopeng

AU - Zachary, Christopher

AU - Shahan, Rachel

AU - Szekley, Pablo

AU - Aardening, Ziv

AU - Vanhoutte, Isabelle

AU - Ma, Qian

AU - Pinto, Lucrezia

AU - Krupař, Pavel

AU - German, Nathan

AU - Zhang, Jingyuan

AU - Simon--Vezo, Claire

AU - Perez-Sancho, Jessica

AU - Quijada, Pepe Cana

AU - Zhou, Qianzi

AU - Lee, Laura R.

AU - Cai, Jianghua

AU - Bayer, Emmanuelle M.

AU - Fendrych, Matyáš

AU - Truernit, Elisabeth

AU - Zhou, Yu

AU - Savaldi-Goldstein, Sigal

AU - Wabnik, Krzysztof

AU - Nolan, Trevor M.

AU - Russinova, Eugenia

PY - 2025/4/17

Y1 - 2025/4/17

N2 - Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis root, we reveal that brassinosteroid activity fluctuates throughout the cell cycle, decreasing during mitotic divisions and increasing during the G1 phase. The post-mitotic recovery of brassinosteroid activity is driven by the intrinsic polarity of the mother cell, resulting in one daughter cell with enhanced brassinosteroid signaling, while the other supports brassinosteroid biosynthesis. The coexistence of these distinct daughter cell states during the G1 phase circumvents a negative feedback loop to facilitate brassinosteroid production while signaling increases. Our findings uncover polarity-guided, uneven mitotic divisions in the meristem, which control brassinosteroid hormone activity to ensure optimal root growth.

AB - Brassinosteroid hormones are positive regulators of plant organ growth, yet their function in proliferating tissues remains unclear. Here, through integrating single-cell RNA sequencing with long-term live-cell imaging of the Arabidopsis root, we reveal that brassinosteroid activity fluctuates throughout the cell cycle, decreasing during mitotic divisions and increasing during the G1 phase. The post-mitotic recovery of brassinosteroid activity is driven by the intrinsic polarity of the mother cell, resulting in one daughter cell with enhanced brassinosteroid signaling, while the other supports brassinosteroid biosynthesis. The coexistence of these distinct daughter cell states during the G1 phase circumvents a negative feedback loop to facilitate brassinosteroid production while signaling increases. Our findings uncover polarity-guided, uneven mitotic divisions in the meristem, which control brassinosteroid hormone activity to ensure optimal root growth.

KW - brassinosteroids

KW - cell cycle

KW - cell division

KW - cell polarity

KW - live-cell imaging

KW - root meristem

KW - single-cell RNA sequencing

UR - https://www.scopus.com/pages/publications/86000636557

U2 - 10.1016/j.cell.2025.02.011

DO - 10.1016/j.cell.2025.02.011

M3 - 文章

C2 - 40068682

AN - SCOPUS:86000636557

SN - 0092-8674

VL - 188

SP - 2063-2080.e24

JO - Cell

JF - Cell

IS - 8

ER -

Polarity-guided uneven mitotic divisions control brassinosteroid activity in proliferating plant root cells

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Keywords

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