Single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies

Hee Jin Jeong; Jinhua Dong; Hiroshi Ueda

doi:10.3390/s19010052

Single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies

Hee Jin Jeong
, Jinhua Dong
, Hiroshi Ueda

Research output: Contribution to journal › Article › peer-review

26 Scopus citations

Abstract

We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.

Original language	English
Article number	52
Journal	Sensors (Switzerland)
Volume	19
Issue number	1
DOIs	https://doi.org/10.3390/s19010052
State	Published - 2019
Externally published	Yes

Keywords

H1N1
Hemagglutinin
Immunosensor
Influenza A
Quenchbody

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10.3390/s19010052

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title = "Single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies",

abstract = "We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.",

keywords = "H1N1, Hemagglutinin, Immunosensor, Influenza A, Quenchbody",

author = "Jeong, \{Hee Jin\} and Jinhua Dong and Hiroshi Ueda",

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AU - Jeong, Hee Jin

AU - Dong, Jinhua

AU - Ueda, Hiroshi

PY - 2019

Y1 - 2019

N2 - We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.

AB - We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.

KW - H1N1

KW - Hemagglutinin

KW - Immunosensor

KW - Influenza A

KW - Quenchbody

UR - https://www.scopus.com/pages/publications/85058908352

U2 - 10.3390/s19010052

DO - 10.3390/s19010052

M3 - 文章

C2 - 30583603

AN - SCOPUS:85058908352

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JO - Sensors (Switzerland)

JF - Sensors (Switzerland)

IS - 1

M1 - 52

ER -

Single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies

Abstract

Keywords

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