Voltage-clamp recordings of postsynaptic currents in substantia gelatinosa neurons in vitro and its applications to assess synaptic transmission

Kun Yang; Yun Qing Li; Eiichi Kumamoto; Hidemasa Furue; Megumu Yoshimura

doi:10.1016/S1385-299X(01)00069-1

Voltage-clamp recordings of postsynaptic currents in substantia gelatinosa neurons in vitro and its applications to assess synaptic transmission

Kun Yang
, Yun Qing Li
, Eiichi Kumamoto
, Hidemasa Furue
, Megumu Yoshimura

Fourth Military Medical University
Saga Medical School
Kyushu University

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

We describe here procedures for recording postsynaptic currents in substantia gelatinosa neurons on a transverse spinal cord slice preparation with an attached dorsal root. At the holding potential of -70 mV, glutamatergic spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root (Aδ and/or C fiber) stimulation-evoked EPSCs could be observed. Whereas at the holding potential of 0 mV, spontaneous inhibitory postsynaptic currents (IPSCs) and dorsal root Aδ fiber stimulation-evoked IPSCs could be encountered. The methods make it possible to evaluate synaptic transmission by analysing the postsynaptic currents on dorsal root attached spinal cord slice.

Original language	English
Pages (from-to)	235-240
Number of pages	6
Journal	Brain Research Protocols
Volume	7
Issue number	3
DOIs	https://doi.org/10.1016/S1385-299X(01)00069-1
State	Published - 2001
Externally published	Yes

Keywords

Excitatory postsynaptic currents (EPSCs)
Inhibitory postnaptic currents (IPSCs)
Paint
Rat
Spinal cord
Substantia gelatinosa

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10.1016/S1385-299X(01)00069-1

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abstract = "We describe here procedures for recording postsynaptic currents in substantia gelatinosa neurons on a transverse spinal cord slice preparation with an attached dorsal root. At the holding potential of -70 mV, glutamatergic spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root (Aδ and/or C fiber) stimulation-evoked EPSCs could be observed. Whereas at the holding potential of 0 mV, spontaneous inhibitory postsynaptic currents (IPSCs) and dorsal root Aδ fiber stimulation-evoked IPSCs could be encountered. The methods make it possible to evaluate synaptic transmission by analysing the postsynaptic currents on dorsal root attached spinal cord slice.",

keywords = "Excitatory postsynaptic currents (EPSCs), Inhibitory postnaptic currents (IPSCs), Paint, Rat, Spinal cord, Substantia gelatinosa",

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T1 - Voltage-clamp recordings of postsynaptic currents in substantia gelatinosa neurons in vitro and its applications to assess synaptic transmission

AU - Yang, Kun

AU - Li, Yun Qing

AU - Kumamoto, Eiichi

AU - Furue, Hidemasa

AU - Yoshimura, Megumu

PY - 2001

Y1 - 2001

N2 - We describe here procedures for recording postsynaptic currents in substantia gelatinosa neurons on a transverse spinal cord slice preparation with an attached dorsal root. At the holding potential of -70 mV, glutamatergic spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root (Aδ and/or C fiber) stimulation-evoked EPSCs could be observed. Whereas at the holding potential of 0 mV, spontaneous inhibitory postsynaptic currents (IPSCs) and dorsal root Aδ fiber stimulation-evoked IPSCs could be encountered. The methods make it possible to evaluate synaptic transmission by analysing the postsynaptic currents on dorsal root attached spinal cord slice.

AB - We describe here procedures for recording postsynaptic currents in substantia gelatinosa neurons on a transverse spinal cord slice preparation with an attached dorsal root. At the holding potential of -70 mV, glutamatergic spontaneous excitatory postsynaptic currents (EPSCs) and dorsal root (Aδ and/or C fiber) stimulation-evoked EPSCs could be observed. Whereas at the holding potential of 0 mV, spontaneous inhibitory postsynaptic currents (IPSCs) and dorsal root Aδ fiber stimulation-evoked IPSCs could be encountered. The methods make it possible to evaluate synaptic transmission by analysing the postsynaptic currents on dorsal root attached spinal cord slice.

KW - Excitatory postsynaptic currents (EPSCs)

KW - Inhibitory postnaptic currents (IPSCs)

KW - Paint

KW - Rat

KW - Spinal cord

KW - Substantia gelatinosa

UR - https://www.scopus.com/pages/publications/0034954519

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DO - 10.1016/S1385-299X(01)00069-1

M3 - 文章

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AN - SCOPUS:0034954519

SN - 1385-299X

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JO - Brain Research Protocols

JF - Brain Research Protocols

IS - 3

ER -

Voltage-clamp recordings of postsynaptic currents in substantia gelatinosa neurons in vitro and its applications to assess synaptic transmission

Abstract

Keywords

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