岩藻黄素纳米复合物的制备及抗氧化、抗肿瘤活性研究

Fucoxanthin, a liposoluble carotenoid, has a limited application in food industry due to poor water solubility and strong environmental sensitivity. In the paper, high-purity fucoxanthin was prepared by organic solvent extraction in macroporous resin column chromatography form Undaria pinnatifida; then, fucoxanthin loaded whey protein isolated (WPI)-gum Arabic (GA) nanocomplexes were developed using complex coacervation. The nanocomplexes presented a smooth spherical shape with a diameter of about 44nm under TEM. The Fourier Transform infrared spectroscopy show that the typical peak of allenic bond and acetyl group vanished, indicating there was interaction between fucoxanthin and WPI/GA. The fucoxanthin nanocomplexes showed a higher antioxidant activity stability than free fucoxanthin. In addition, anti-tumor assay showed that the nanocomplexes retained the biological activity of fucoxanthin to inhibit the proliferation of HT29 cells.