Corrigendum to “The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides” [Carbohydrate Polymers Volume 276(2022), 118699] (Carbohydrate Polymers (2022) 276, (S0144861721010869), (10.1016/j.carbpol.2021.118699))

The authors regret to add the Supplemental Information to “The anti-inflammatory activity of specific-sized hyaluronic acid oligosaccharides”. Supplemental Information Materials and methods 1. Cytotoxicity of ΔHA2 in RAW264.7 cells MDA-MB-231 cells were incubated in 96-well polystyrene cell culture plates for 24 h. The cells were incubated with ΔHA for 48 h at 37 °C. Afterwards, the cells were incubated for 4 h with 2 mg/mL resazurin solution at 37 °C, and then the fluorescence intensity was measured using a SpectraMax M3 microplate reader (Molecular Devices, San Jose, CA, USA) at an excitation wavelength of 544 nm and emission wavelength of 595 nm. 2. Molecular Docking Molecular docking was performed by using Molecular Operating Environment (MOE) software (2015.10, Chemical Computing Group, Montreal, Canada). The X-ray diffraction crystal structures of mouse TLR4 (ID: 2Z64) were obtained from the Protein Data Bank. There is similarity in binding of HA between mouse and human active sites. Three-dimensional structures of ΔHA were generated by MOE. The protein structures were prepared using the QuickPrep module of MOE, and the energies were minimized through the General method at a 0.1 kcal mol⁻¹A°⁻² Root Mean Square (RMS) Gradient. The docking poses were scored using a London 4G scoring function with five parameters including rotational and translational entropy, ligand flexibility, hydrogen bonding, metal ligations, and desolvation energy. [Table presented] [Table presented] [Table presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] [Figure presented] The authors would like to apologise for any inconvenience caused.