Riboflavin-protected ultrasmall silver nanoclusters with enhanced antibacterial activity and the mechanisms

Developing silver nanomaterials with efficient antimicrobial properties is of importance for combating bacteria. Here, we report ultrasmall riboflavin-protected silver nanoclusters (RF@AgNCs) that can effectively kill or suppress the growth of Gram-positive S. aureus, Gram-negative E. coli, and fungi C. albicans. Riboflavin (RF) with intrinsic biocompatibility was used as a surface ligand to synthesize silver nanoclusters. TEM revealed that the synthesized RF@AgNCs were ultrasmall (2.4 ± 1.2 nm), spherical and well-dispersed. Antibacterial activity tests showed that RF@AgNCs possessed superior antibacterial efficacy in comparison with RF, AgNPs and mixed RF and AgNPs (RF + AgNPs). The mechanisms of antibacterial activity of RF@AgNCs were studied by fluorescence microscopy-based Live/Dead cell staining assays and ROS measurement. And the results illustrated that the integrity of the bacteria membrane was disrupted and intracellular high level ROS generation was induced by RF@AgNCs. The cytotoxic activities were also assessed and RF@AgNCs were found to be non-toxic to human red blood cells and mammalian cells. With the highly efficient antibacterial activity and acceptable biocompatibility, RF@AgNCs hold great promise in biomedical applications as well as in water sterilization and the textile industry.