Single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies

Hee Jin Jeong; Jinhua Dong; Hiroshi Ueda

doi:10.3390/s19010052

Single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies

Hee Jin Jeong
, Jinhua Dong
, Hiroshi Ueda

Hongik University
Weifang Medical University
Institute of Science Tokyo

科研成果: 期刊稿件 › 文章 › 同行评审

26 引用（Scopus）

摘要

We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.

源语言	英语
文章编号	52
期刊	Sensors (Switzerland)
卷	19
期	1
DOI	https://doi.org/10.3390/s19010052
出版状态	已出版 - 2019
已对外发布	是

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abstract = "We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.",

keywords = "H1N1, Hemagglutinin, Immunosensor, Influenza A, Quenchbody",

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AU - Jeong, Hee Jin

AU - Dong, Jinhua

AU - Ueda, Hiroshi

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N2 - We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.

AB - We have successfully generated a Quenchbody that enables the detection of the influenza virus hemagglutinin (HA), in a simple and convenient manner. By two-site labeling of the bacterially-produced anti-HA Fab with ATTO520, its fluorescence intensity was increased to 4.4-fold, in the presence of a nanomolar concentration of H1N1 HA. Our results indicate the potential use of this Quenchbody, as a sensor for the simple in situ detection of influenza A virus.

KW - H1N1

KW - Hemagglutinin

KW - Immunosensor

KW - Influenza A

KW - Quenchbody

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Single-step detection of the influenza virus hemagglutinin using bacterially-produced quenchbodies

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